Fig. 4. Effects of disease-associated mutations S54F, 95-99 and P183H on emerin binding to BAF and lamin A. (A) Bacterial lysates containing wildtype (wt) emerin protein, disease-linked emerins (S54F, 95-99, P183H) or alanine-substitution mutants (m24 and m141) were separated on gels, blotted and probed with ³⁵S-labeled BAF or ³⁵S-labeled lamin A. (B) Wildtype and mutant emerin proteins were synthesized as ³⁵S-labeled proteins in vitro, mixed with ³⁵S-labeled BAF, and immunoprecipitated with immune (shown) or preimmune (not shown) antibodies against emerin (see Materials and Methods).