
Fig. 6. Localization of GFP-fused emerin mutants S54F,
95-99, P183H and P183T in living HeLa cells. HeLa cells were transiently transfected to express the indicated emerin mutant as a GFP-fusion protein. (A) GFP fluorescence during interphase. (B) GFP fluorescence in living cells 5-7 minutes after the metaphase-anaphase transition, when wildtype emerin localizes to the core region of telophase chromosomes. Bars, 10 µm.