Fig. 3. Molecular domains of emerin necessary for its localization. (a) Molecular structure of emerin and its truncations. GFP was fused to their C-terminus. Localization of each truncation at the NE and the ‘core’ is summarized: N-terminal 65 residues are necessary and sufficient for the ‘core’ localization of emerin; C-terminal domain is required for nuclear membrane localization. Examples are shown for 66-254 (b-e), for 1-64 (f,g), for 226-254 (h-k), and for 175-254 (l-o). In (g) time-lapse images at 1 minute intervals are shown. Bar, 10 µm.