Fig. 4. Intracellular localization of BAF during mitosis. (a) HeLa cells stained with antibodies #3273 against human BAF (see Methods). b HeLa cells stained with anti-BAF peptide antibodies (Furukawa, 1999). c HeLa cells that express GFP-BAF and stained with antibodies against emerin. d Time-lapse images of a living HeLa cell that expresses GFP-BAF. Chromosomes were stained with DAPI in fixed cells, and with Hoechst 33342 in living cells. Bar, 10 µm.