Fig. 10. Glycosaminoglycan sulfation is not required for fibulin-1 inhibition of FN-stimulated haptotactic migration. MDA MB231 cells were cultured in the absence or presence of chlorate (20 mM) for 18 hours in low-sulfate HAMS/F12 medium and 10% dialysed BCS. (A) Chlorate treatment inhibited 98% of the normal amount of [³⁵S]-SO₄ incorporated into proteoglycans in the MDA MB231 cell layer. (B) Fibulin-1 inhibited the FN-stimulated haptotactic migration of both the control and chlorate-treated MDA MB231 cells to a similar extent.