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Fig. 7. {alpha}4-Integrins are not required for fibulin-1 inhibition of FN-stimulated haptotactic migration. (A) Evaluation of the effect of fibulin-1 on haptotactic migration of CHO cells engineered to express {alpha}4ß1. Analysis of expression of the integrin {alpha}4 subunit in various cultured lines by RT-PCR (B) and immunoblotting (C). The arrowheads in C indicate the intact ~150 kDa and ~180 kDa forms of the {alpha}4 polypeptide typically observed under non-reducing conditions.