Fig. 8. Fibulin-1 does not bind to cell surface proteins and does not effect cell surface ß₁ integrin binding to FN. Radiolabelled MDA MB231 cell surface proteins were bound to magnetic beads conjugated with fibulin-1, FN or BSA. Before incubation with cells, FN-beads were incubated with either fibulin-1 (FN + fibulin-1) or BSA (FN + BSA). (A) Autoradiograph and (B) immunoblot analysis using anti-ß₁-integrin cytoplasmic domain antibody.