Fig. 8. Yeast two-hybrid interaction of the N-terminus of GLUT4 with µ-adaptins. (A) Yeast cells (strain HF7c) cotransformed with plasmids for GAL4-BD/GLUT4-NTs and GAL4-AD/µ-adaptins were plated onto drop-out agar plates lacking tryptophane and leucine with (+) and without (–) histidine and incubated for 2 days at 30°C. (B) Quantification of ß-galactosidase activity. Yeast cells (strain SFY526) cotransformed with plasmids for GAL4-BD/GLUT4-NTs and GAL4-AD/µ-adaptins were grown into mid-log phase, harvested and assayed for ß-galactosidase activity using CPRG as substrate (see Materials and Methods).