Fig. 10. Analysis of IKK expression and IKK activity in normal prostate cells and PC cell lines. (A) Western blot analysis of IKK and IKKß expression. Western blots containing whole cell protein extracts from untreated cells (10 µg/lane) were probed for expression of IKK and IKKß. (B) Analysis of IKK activity. Protein extracts from untreated cells were immunoprecipitated with a combination of IKK and IKKß antisera, and used for in vitro kinase reaction. Protein extract from LNCaP cells stimulated by TNF- (7.5 ng/ml, 10 minutes) was used as a positive control.