Fig. 11. Effect of d.n. IKK mutants on NF-B constitutive activity in normal prostate cells and the PC3 cell line. (A) Prostate cells were co-transfected with B-luciferase, pRL-null and (1) control vector; (2) IKK d.n. mutant; (3) IKKß d.n. mutant; and (4) IB d.n. mutant. (B) Prostate cells were co-transfected with MMTV-luciferase, pRL-null and (1) control vector; (2) IKK d.n. mutant; (3) IKKß d.n. mutant; and (4) IB d.n. mutant. Luciferase activity was measured 24 hours after transfection in untreated prostate cells by dual luciferase assay. Data are shown as FL/RL luciferase activities ratio (% to control) for one representative experiment. PrEC, normal epithelial prostate primary cultures.