(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)


Fig. 12. Effect of I{kappa}B{alpha} d.n. mutant on apoptosis in prostate cells. (A) Western blot detection of PARP cleavage. PC3 and LNCaP prostate cells were infected with adenovirus expressing GFP and I{kappa}B{alpha} mutant lacking Ser32 and Ser36 (AdV-d.n.I{kappa}B{alpha}) or adenovirus expressing only GFP (AdV-control). 24 hours later cell cultures were left untreated or treated with TNF-{alpha} (7.5 ng) for 10 hours. PARP cleavage was detected by western blotting with antibody that detects the full length PARP (116 kDa) and PARP cleavage product (85 kDa). Adherent cells and detached floaters were combined for whole-cell lysate preparations. (1) Untreated cells; (2) AdV-d.n.I{kappa}B{alpha}-infected cells; (3) AdV-d.n.I{kappa}B{alpha}-infected cells treated with TNF-{alpha}; (4) AdV-control-infected cells; (5) AdV-control-infected cells treated with TNF-{alpha}. (B) Effect of I{kappa}B{alpha} d.n mutant on morphology of PC3 cells. Micrographs (x300) depicting representative morphological response of PC3 cells 48 hours after infection: (1) with AdV-control; (2) with AdV-d.n.I{kappa}B{alpha}; and (3) with AdV-d.n.I{kappa}B{alpha} and treated with TNF-{alpha}. Note numerous blebbing cells in cell cultures treated with TNF-{alpha}.