Fig. 2. Analysis of constitutive NF-B transcriptional activity in human primary prostate cells and PC cell lines. (A) Constitutive activity of B-luciferase reporter. Prostate cells were cotransfected with B-luciferase reporter and pRL-null construct. Luciferase activity was measured 24 hours after transfection in untreated prostate cells by dual luciferase assay. Data are shown as FL/RL ratio for one representative experiment. (B) Northern blot analysis of constitutive IB and IL-6 genes expression. Northern blots containing total RNA (20 µg/lane) from untreated normal prostate and PC cells were probed for expression of IB and IL-6 genes. The membranes were also hybridized with a 7S RNA probe as a control for equal RNA loading.