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Fig. 3. Western blot analysis of NF-{kappa}B and I{kappa}B protein expression in human primary prostate cells and PC cell lines. Western blots containing whole cell protein extracts (10 µg/lane) from untreated cells were probed for expression of p50, p65, I{kappa}B{alpha}, I{kappa}Bß and I{kappa}B{epsilon}.