Fig. 6. The rate of IB phosphorylation in androgen-dependent and androgen-independent PC cell lines. Indicated cell lines were treated with proteasomal inhibitor MG132 (7.5 µg/ml) for 0.5-6 hours. (A) Western blots containing cytosol proteins (10 µg/lane) were probed for expression of IB-P. (B) Western blots shown in A are plotted as a percentage of the maximum IB-P expression level. Abscissa: time after MG132 treatment (hours). Ordinate: relative amount of IB-P. Data are shown for the one representative experiment.