Fig. 7. The rate of IB-P degradation is higher in androgen-independent PC cells. Indicated cell lines were treated with cycloheximide (CHX, 10 µg/ml) in combination with 15-deoxy-^12,14-prostaglandin J2 (15dPGJ2) for 0.5-6 hours. (A) Western blots containing cytosol protein extracts (10 µg/lane) were probed for expression of IB-P. (B) Western blots shown in A are plotted as a percentage of the initial IB-P expression level. Abscissa: time after treatment (hours). Ordinate: relative amount of IB-P. Data are shown for the one representative experiment.