Fig. 8. Effect of cycloheximide on NF-B binding in PC cells. Indicated cell lines were treated with CHX (10 µg/ml) for 0.5-3 hours. Nuclear proteins (5 µg/reaction) from cells were used for electrophoretic mobility shift assay (EMSA). (A) NF-B binding activity. (B) NF-B binding activity shown in A is plotted as a percentage of the maximum (at 3 hours of CHX treatment) p65/p50 binding level. Abscissa: time after treatment (minutes). Ordinate: relative amount of NF-B binding. Data are shown for the one representative experiment.