Fig. 5. Anti-apoptotic effects of overexpressed Rad51 protein. (A) Simultaneous staining of etoposide-treated TGR928.1-9 cells with annexin V (green) and anti-Rad51 antibodies (red). Annexin-V-positive apoptotic cells are Rad51-foci-negative. (B) Reduced frequency of apoptotic cells in etoposide- and UV-treated Rad51-overexpressing cultures. Cells staining positively for annexin V (annexin+) were scored as apoptotic, whereas non-apoptotic cells were annexin–. Simultaneously, the cells were stained for the presence (Rad51+) or absence (Rad51–) of Rad51 foci. At least 400 cells were analyzed for each experiment.