Fig. 2. Time course of receptor recycling. After induction of receptor sequestration for 15 minutes with 100 nM NT, the peptide was removed by acid washes and the amount of cell surface receptor was determined at indicated times of incubation at 37°C as described in Materials and Methods. (A) Recycling of the wild-type (WT) rNTR1 (n=4) (closed squares), mNTR2-WT (n=6) (closed circles) and effect of the recycling inhibitor monensin on the mNTR2-WT (n=3) (open circles). (B) Recycling of the mNTR2-WT in the absence of drug (closed circles) (n=6) or in the presence of genistein (closed inverted triangles) (n=2) or brefeldin A (BFA) (open diamonds) (n=2), and of the Y237A-mNTR2 mutant (inverse open triangles) (n=4).