Fig. 2. Ang2 induces chemotaxis, but not proliferation of IBE cells. (A) Cells were inoculated into fibronectin-coated wells and cultured in Ham’s F-12 medium containing 10% FBS. On the following day, medium was replaced with Ham’s F-12 medium containing 0.25% BSA with (closed bar) or without (open bar) 5 ng/ml FGF-2 in the presence or absence of Ang2 and the culture continued for 3 days. Data are expressed as means±s.d. for triplicated wells and similar results were obtained from two independent experiments. The number of cells counted in untreated samples was set as 100%. (B) Cells were suspended in Ham’s F-12 medium containing 0.25% BSA and seeded onto the upper surface of a fibronectin-coated Traswell membrane. In the lower wells, 50 ng/ml FGF-2 was added to the same medium (indicated as closed bars) in combination with Ang2. Four hours later, cells attached on the lower surface of the membranes were counted. Data are expressed as means±s.d. for quadruplicated wells and similar results were obtained from two independent experiments. The number of cells counted in untreated samples was set as 100%.