Fig. 5. Stability of Sds22p and Glc7p in the sds22-6 mutant. Strains solely dependent on myc epitope-tagged wild-type (Sds22p-myc3: SAY326) or mutant (Sds22-6p-myc3: SAY284) Sds22p or wild-type (MPY1165) and sds22-6 (MPY1171) strains expressing HA epitope-tagged Glc7p (HA-Glc7p) were shifted to 37°C and the stability of either protein monitored by western blot analysis over 6 hours. The level of calmodulin (Cmd1p) is shown to demonstrate equivalent loading of each lane and the growth curve indicates cell density at each time point.