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Fig. 8. Glc7p localization is affected in the sds22-6 mutant. Diploid SDS22/SDS22 (KT2070), sds22-6/sds22-6 (KT2066) and SDS22/sds22-6 (KT2067) cells expressing GFP-Glc7p were grown at 22°C and then shifted to 37°C. The effect of the temperature shift on wild-type and mutant cells is presented for a representative field, showing DIC and GFP fluorescence images for each time point.