Fig. 2. C1D and TRAX interact in vitro. C1D and hepatitis C virus core proteins (HCV-core/pQE) were expressed in bacteria using the plasmid pQE30 (Qiagen) and purified by Ni-NTA column under denaturing conditions. One µg of each of the immobilized fusion proteins and pQE alone were incubated with In vitro Transcribed and Translated (IVTT) TRAX product and pull-down assay was performed. One tenth of the input TRAX was bound by C1D.