Fig. 5. SDS-resistant C1D homodimers interact with TRAX upon -irradiation. (A) Transiently transfected COS 7 cells were immunoprecipitated using the antibodies indicated. For lanes 3-6 the cells were treated with 20 Gy of ionizing irradiation before lysate preparation. Western blotting was performed by an anti-C1D antibody. The monomer and dimer forms of C1D are indicated. (B) Transiently transfected COS 7 cells were immunoprecipitated using the indicated antibodies. For lane 3-7, the cells were treated with 50 j/m² UV irradiation. The top panel shows the western blotting using an anti-C1D antibody, and in the bottom panel, the blot was stripped and reprobed with an anti-GFP antibody to demonstrate the presence of GFP-TRAX. For each lane, immunoprecipitations were performed using the cell lysates obtained from 3x10⁵ cells.