Fig. 1. The GFP-tagged uracil permease is correctly delivered to the plasma membrane. Wild-type cells transformed with p197gF or p195gF-GFP (A) or pFl38gF-GFP (B) and grown on lactate as a carbon source were induced by growth for two hours on galactose medium. (A) Uracil permease activity was followed at various times after addition of galactose. (O) and () corresponded to cells transformed with p197gF and p195gF-GFP respectively. (B) Cell fluorescence was followed at various times after addition of galactose. Fluorescence staining was only observed in budding cells. Cells were viewed with a FITC filter set.