Fig. 5. Fur4p-GFP partially colocalizes with Pep12p in yck^ts cells. yck^ts cells transformed with pFL38gF-GFP were grown to exponential growth phase at 24°C in galactose medium. Cells were incubated at 37°C for 20 minutes. The synthesis of Fur4p-GFP was stopped by adding glucose and incubating for 10 minutes at the same restrictive temperature. Cells were observed 90 minutes after the addition of cycloheximide at 37°C. Cells were fixed and processed for immunofluorescence using the anti-Pep12p antibody followed by rhodamine-labeled goat anti-mouse IgG. GFP fluorescence was visualized with a FITC filter and Pep12p distribution was observed with a rhodamine filter. Regions of colocalization are shown in yellow on the overlay.