Fig. 1. Direct chemotactic effects of immunopurified AT on monocytes and lymphocytes. Chemotaxis experiments were performed in modified Boyden chambers using nitrocellulose micropore filters. Monocyte chemo-attractant protein 3 (MCP-3) for monocytes (left panel) and interleukin-8 (IL-8) for lymphocytes (right panel) were used as positive control chemotactic stimuli. Results are given as the mean±s.e.m. of the migration index, which is the ratio of the distance of migration (µm) towards a chemotactic agent and the distance (µm) towards the medium. Similar results were obtained using AT concentrate (data not shown). Multiple group comparison was performed after Kruskal-Wallis (p=0.0074 for lymphocytes, p=0.0001 for monocytes) followed by Mann-Whitney U-test (*, p<0.05). n=8 for lymphocytes, n=5 for monocytes.