Fig. 6. FACS and RT-PCR analyses of syndecan-4 in lymphocytes and monocytes. (A) FACS analysis of anti-syndecan-4 mAb binding to lymphocytes and monocytes. Fluorescence analysis used a FACScan Flow cytometer, and a histogram of PE-fluorescence is shown. Cells were preincubated with either 10 µg/mL isotype-matched control mouse IgG (thin line) or 10 µg/mL anti-syndecan-4 mAb (bold line) and stained with PE-conjugated streptavidin. (B) Syndecan-4 mRNA in endothelial cells, lymphocytes and monocytes. 1 µg of total RNA from each sample was reverse transcribed into cDNA and amplified for the syndecan-4 gene using PCR. Syndecan-4 is represented by the 453 base pair product.