Fig. 7. Effects of syndecan-4 antibodies on AT-induced chemotaxis of monocytes and lymphocytes. Preincubation with antibodies to syndecan-4 core protein (D-16) and syndecan-4 ectodomain (5G9) was performed for 20 minutes (37°C/5% CO₂). After washing, cells were allowed to migrate toward AT [1 U/mL] or Rantes [10 ng/mL] in modified Boyden chambers using nitrocellulose micropore filters. Isotype-matched IgG served as control. Response of monocytes (A) and lymphocytes (B) is expressed using the chemotaxis index (mean±s.e.m.), which is the ratio between directed and undirected migration of cells. The distance of undirected migration was 68±4.9 µm for monocytes and 57±9.5 µm for lymphocytes (n=4). Statistical analysis was performed using the Mann-Whitney U test (*, p<0.05) versus medium after Kruskal-Wallis (p<0.01), n=4. Chemotaxis toward Rantes remained unaffected after pre-treatment with syndecan-4 antibodies (C). Kruskal-Wallis (p>0.1)