Fig. 11. Absence of, and reduced staining of laminin-1 in tumor-associated myoepithelial cells. Double-labeling immunofluorescence of a normal acinus in a TDLU (a,b) and two different carcinomas (c,d and e,f) to demonstrate laminin-1 in red (a,c,e) and in a coexposure with myoepithelial cells in green (b,d,f) as stained with keratin 17 (b,d) or maspin (f). The bottom panels illustrate the presence of myoepithelial cells at the stromal (S)-cancer epithelial (C) interface in total absence of laminin-1 staining. The middle panels show infiltrating ductal carcinoma with tumor-associated myoepithelial cells expressing a variable but generally weak staining for laminin-1 compared with a normal acinus in the top row. (bar, 50 µm).