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Fig. 5. LAMA-1 chain is expressed only in myoepithelial cells. RT-PCR analysis of laminin {alpha}1 (LAMA1), laminin {alpha}3 (LAMA3), laminin {alpha}5 (LAMA5), type IV collagen {alpha}1 (COL4A1) and type IV collagen {alpha}2 (COL4A2) in luminal epithelial cells in rBM and collagen-I gel and in myoepithelial cells and MCF-7 cells. GAPDH is used as an internal control. Note that the only laminin chain that is not expressed in the pure luminal epithelial cultures is the {alpha}1-chain.