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Fig. 8. The cancer-derived myoepithelial cell line lacks the expression of laminin-{alpha}1 chain only. RT-PCR on RNA extracted from cancer-derived myoepithelial cell line, immortalized, normal-derived myoepithelial cells (MEP), primary cultured MEP and MCF-7 S9 cells. The panel shows {alpha}1-chain (LAMA1), {alpha}3-chain (LAMA3), {alpha}5-chain (LAMA5) and GAPDH as an internal control. While the {alpha}3 mRNA was low in the cancer-derived MEP cell line, the {alpha}l-chain was the only differentially expressed chain between normal-derived and cancer-derived myoepithelial cells.