Fig. 3. Subcellular localization of myne-1. Adult mouse skeletal muscle was fractionated and immunoblotted with AM1. The protocol for cell-membrane fractionation is shown in A. The corresponding fractions are shown in lanes 1, 2 and 3. Enrichment of myne-1 is shown in the crude nuclear fraction 3 (lane 3). The protocol for nuclear membrane and nucleoplasm separation is shown in B. The corresponding fractions are shown in lanes 1, 2 and 3. Myne-1 is enriched in the nuclear membrane fraction 3 (lane 3).