
Fig. 5. Colocalization of myne-1, lamin A/C and emerin in skeletal muscle. Transverse sections of quadriceps muscle were stained with AM1 (A,C,D,E,G and H). Staining with lamin A antibody is shown in B, and double staining with AM1 and lamin is shown in C and D. Staining with emerin is shown in F, and double staining with AM1 and emerin is shown in G and H. D shows a higher magnification view of the boxed region in C. Similarly, H shows the boxed region in G at higher magnification. Note colocalization of myne-1 and lamin A appears as yellow staining in C and D. In contrast, in G and H, some nuclei that express myne-1, but not emerin, are shown as red. The arrow in H indicates a cell within the artery wall that expressed myne-1 but not emerin. Low magnification bars represent 100 µm. High magnification bars represent 25 µm.