
Fig. 6. Immunolocalization of myne-1 in differentiating C2C12 cells. A,C,E,G and I represent nuclei stained with DAPI. AM1 labeling is shown in B,D,F,H and J. UN indicates undifferentiated C2C12 cells. D1, D3, D5 andD9 indicate 1 day, 3 days, 5 days and 9 days of differentiation, respectively. During differentiation, myne-1 is localized to discrete intranuclear foci, whereas late in differentiation, it becomes localized to the nuclear membrane. The bar represents 30 µm.