Fig. 8. Co-immunoprecipitation of myne-1 and lamin A/C. Fully differentiated C2C12 cells were lysed and the soluble fraction was processed for immunoprecipitations using myne-1-specific antibody AM1 coupled to Protein A/G sepharose beads. The precipitate was separated by electrophoresis on parallel SDS-PAGE gels. One was stained with Coomassie blue (A), and the second was immunoblotted with a lamin-A/C-specific antibody XB10 (B). Lane 1, total cell lysate; lane 2, lysate precipitated with Protein A/G sepharose beads lacking AM1; lane 3, AM1 immunoprecipitated with Protein A/G sepharose in the absence of lysate; lane 4, lysate immunoprecipitated with AM1 on Protein A/G sepharose beads.