Fig. 3. Phagosomal translocation of p61Hck lysosomal isoform in transfected CHO-CR3. CHO-CR3 cells transiently expressing the lysosomal p61Hck-GFP (A) or cytosolic p61_G2AHck-GFP (B) were incubated with serum-opsonized zymosan. Cells were fixed and non-permeabilized, extracellular zymosan particles were seen with primary Abs revealed by secondary TRITC-conjugated Abs. Translocation of p61Hck-GFP on phagosomes is visualized by a green fluorescent ring (A, arrow) although no fluorescent ring was seen on the phagosomal membrane (black hole) in cells expressing the cytosolic form of Hck (B, arrowhead) as observed by confocal microscopy. Bar, 7 µm.