Fig. 6. Role of phagocytic receptors in the fusion of phagosomes with Hck and CD63 lysosomes. MDMs were incubated with latex beads coated with mannosylated-BSA (Man-BSA-latex) or human IgG (IgG-latex), and phagocytosis was performed under synchronized conditions. Two hours post-infection, cells were fixed and permeabilized with methanol, and double immunostaining was performed using Abs directed against CD63 or Hck. Mannosylated-latex beads resided in phagosomes negative for Hck (A) and positive for CD63 (B) (arrows). IgG-coated latex beads resided in phagosomes positive for Hck (C) and CD63 (D) (arrows). Bar, 8 µm. (E) Recruitment of Hck and CD63 to phagosomes containing IgG-latex beads was quantified as a percentage of the phagosomes positive for each marker at different time points. Data are from one experiment that is representative of four independent experiments.