Fig. 1. Annexin 2 is recruited to sites of adhering EPEC. HeLa cells were infected with EPEC for 3 hours, permeabilized and fixed with PFA. The endogenous annexin 2 was visualized using the monoclonal anti-annexin 2 antibody HH7 and F-actin was stained with rhodamine-conjugated phalloidin. Note that EPEC-infected cells show bright annexin 2 (A) and F-actin (B) signals beneath adhering bacteria (arrows) (a merge of the images is given in C). The enlarged insets show a twofold magnification of the boxed region and reveal that annexin 2 seems to be localized in cuplike structures around the recruited actin. Bar, 10 µm.