Fig. 3. HEF1 localizes to prominent focal adhesion sites. Immunofluorescent staining of either uninduced (A-C) or induced (D-I) HEF1.M1 cells was performed using antisera specific for HEF1 (A,D,G) and antibodies specific for paxillin (B,E) or phalloidin-stained F-actin (H). Merged images (C,F,I) demonstrate the pronounced co-localization of HEF1 and paxillin to the prominent focal adhesion sites in the leading edge lamellipodia (F), with HEF1 present at the distal ends of F-actin-rich stress fibers (I, arrowheads). Images depict 1.4 µm sections, acquired using a Bio-Rad MRC 600 confocal microscope (60x objective). Bar, 25 µm.