Fig. 5. HEF1 production enhances cell motility by increasing cell speed. The average speed of uninduced or induced HEF1.M1 (A) or CM1 (B) cells was determined by analyzing the movement of individual cells at 5-minute intervals over the course of 6 hours using Inovision ISEE^TM nanotracking software. (A) The average speed of uninduced (gray) or induced (black) HEF1.M1 cells, grouped into speed ranges. (B) The average speed of uninduced (gray) or mock induced (black) CM1 cells, grouped into speed ranges; the speed of CM1 cells was not altered by mock induction. These data represent the average speed of HEF1.M1 cells (uninduced, N=59; induced, N=55) and CM1 cells (uninduced, N=76; induced, N=60) derived from two independent experiments.