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Fig. 3. Confocal images of mini-CBs (arrowheads) stained with four antibodies that recognize typical CB components (first column) and counterstained with {alpha}-coilin serum C236 to identify the mini-CBs (second column). The overlay is shown in the third column and a DIC image is shown in the fourth column. In each case, the mini-CB is attached to the surface of a B-snurposome (b). (A) mAb H14, which recognizes the largest subunit of Pol II when the C-terminal domain is phosphorylated on serine 5. Only the mini-CB is stained. (B) mAb K121 against the trimethylguanosine cap found on several snRNAs stains the mini-CB and two B-snurposomes. (C) mAb Y12 against the Sm epitope on snRNPs stains the mini-CB and several B-snurposomes. (D) mAb No114 against Xenopus Nopp140 stains the mini-CB and the nucleolus (n) but not the B-snurposome.