Fig. 4. A small fraction of the contents of a single GV from an oocyte that was heat shocked at 31.5°C for 3 hours, injected with GFP-coilin transcripts (into the cytoplasm) and allowed to recover overnight. (A) Immunofluorescence after staining with an anti-GFP antibody to show the localization of GFP-coilin in mini-CBs. The anti-GFP antibody enhanced the detectable but weak signal from mini-CBs. (B) DIC image of the same field. Mini-CBs are indicated by arrowheads. The larger round objects are B-snurposomes; a single nucleolus (n) is also present.