Fig. 6. Western blot of three phosphorylated proteins from GVs of control oocytes (-hs), oocytes heat shocked at 31.5°C for 4 hours (+hs) and oocytes allowed to recover overnight at 18°C after heat shock (hsR). Immunostained with mAb ARNA-3 against the largest subunit of RNA PolII, mAb No114 against Xenopus Nopp140 and mAb H1 against Xenopus coilin. No obvious differences in mobility were observed that might have indicated a change in phosphorylation state.