Fig. 2. Subcellular localization of h1 CaP constructs in A7r5 cells. Both h1 CaP and h1 CaPt localize preferentially to the central portion of the stress fibers. Note the lack of overlap for EGFP (green) and phalloidin (red) signals at the ends of stress fibers, corresponding to sites of focal adhesions (arrows). Disruption of the ABS1 by site-directed mutagenesis (h1ABS1-k.o.) causes a significant change in the localization pattern. This effect is reversed by the additional deletion of the tail (h1t ABS1-k.o.). Top row, actin visualized by Alexa 568 phalloidin; middle row, GFP fluorescence; bottom row, merged images.