Fig. 4. Subcellular localization of h2 CaP constructs in A7r5 cells. Deletion of the negatively charged tail sequence in h2 CaP (h2t) causes a prominent shift in subcellular localization from a more general actin-binding, to a strictly central stress-fiber-associated pattern. Neutralization of acidic residues by their uncharged counterparts (h2 ch-k.o.) has an effect on actin binding and localization similar to that seen following complete elimination of Thr tail sequences. Top row, actin visualized by Alexa 568 phalloidin; middle row, GFP fluorescence; bottom row, merged images.