Fig. 1. Immunoblot identification of accumulation of a 160 kDa protein with
FN-coated beads. Unbound fractions (lane 1), bead-bound protein complexes
(lane 2) from either ConA- or FN-coated beads, and rat brain extract (10
µg) were electrophoresed on an SDS-containing 4-12% polyacrylamide gel
under reducing conditions and transferred to nitrocellulose. The membrane was
probed with a putative PKC
monoclonal antibody. Indicated on the right
are the molecular masses (kDa) of marker proteins.