Fig. 3. Kinectin is specifically associated with adhesion complexes induced by FN-coated beads and ₅ß₁ integrin. (A) Bead-associated protein complexes induced by FN, fibronectin fragment III_5-10, or mutant fragment III_5-10KGE. (B) Bead-associated protein complexes were induced by mAb13 against the ß₁ integrin or control antibody ES66. (C) Concanavalin A-coated (ConA) or fibronectin-coated (FN) beads were either mixed directly with HFF homogenates (Lysate) or incubated with intact, living HFF (Cells). Bead-bound protein complexes were then isolated, separated on 4-12% SDS-containing polyacrylamide gels, transferred to nitrocellulose, and probed with kinectin KR160.9 monoclonal antibody. Note that kinectin is present only in the integrin-based adhesion complexes.