Fig. 5. Cellular localization of kinectin. Human fibroblasts were allowed to spread for 30 minutes on fibronectin-coated coverslips, fixed and double-stained for (A) kinectin (a,a') and -actinin (b,b') or (B) kinectin (a,a') and F-actin (b,b'). The green and the red signals are merged in panels c and c', and cell morphology is shown in the phase-contrast images in panels d and d'. Arrows point to areas that are enlarged on panels a' to d'. Note that kinectin is observed not only within the perinuclear part of the cell body, but also in fibril-like patterns that follow the actin cytoskeleton. Bar, 5 µm.