Fig. 6. Fibronectin-induced clustering of kinectin and cytoskeletal proteins. Immunofluorescence staining of HFF clustered with either FN- (A,C,E,G,I,K) or polylysine-coated beads (B,D,F,H,J,L). Each inset shows a higher magnification that focuses on the equator of the bead marked by the arrowhead. After 20 minutes incubation with beads, cells were fixed and stained with antibodies against kinectin (A,B), calreticulin (C,D), RAP (E,F), paxillin (G,H), ß₁ integrin (I,J), or tubulin (K,L) as described in Materials and Methods. Bar, 20 µm.