Fig. 7. Binding of biotinylated sperm GSTs to components of goat ZP. Glycoproteins of goat ZP on SDS-PAGE under reducing conditions separate into three bands (lane a). Lane b shows the binding of biotinylated sperm plasma membrane GSTs to band three of goat ZP. Lane c shows the binding of monoclonal antibody MA 451, raised against a ZP 3 peptide from a conserved region, to band three of goat ZP. m, marker.